Optimised RNA extraction method unlocks tough tissues for aquaculture research
Researchers at the University of Edinburgh have optimised a protocol that allows previously tough-to-reach genetic…
Browsing: RNA Extraction
A versatile nuclei extraction protocol for single nucleus sequencing in non-model species
The use of single cell sequencing technologies has exploded over recent years, and is now…
GRAB-ALL – a high-throughput pipeline for DNA/RNA/small RNA purification from tissue samples for sequencing
High-throughput total nucleic acid (TNA) purification methods based on solid-phase reversible immobilization (SPRI) beads produce…
A novel cell membrane-associated RNA extraction method
Cell membrane-associated RNA (mem-RNA) has been demonstrated to be cell-specific and disease-related and are considered…
High purity and integrity RNA from human cell lines stored in liquid nitrogen for over 20 years
Biobanks are a key resource for obtaining human cell lines for biomedical research, including for…
RNA extraction method impacts quality metrics and sequencing results in FFPE samples
Archived formalin-fixed, paraffin-embedded (FFPE) tissue samples are being increasingly used in molecular cancer research. Compared…
Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing
Our understanding of nerve regeneration can be enhanced by delineating its underlying molecular activities at…
A protocol for single nucleus RNA-seq from frozen skeletal muscle
Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information…
Isolation of high-quality total RNA from small animal articular cartilage for RNA-seq analysis
Researchers from the University of Debrecen highlight the importance of obtaining high-quality RNA for RNA-seq…
DeLTa-Seq – direct-lysate targeted RNA-Seq from crude tissue lysate
Quantification of gene expression such as RNA-Seq is a popular approach to study various biological…