RNA sequencing (RNA-seq) has revolutionized our ability to study gene expression by providing detailed snapshots of RNA molecules within cells. In the context of single-cell and single-nucleus RNA sequencing, researchers face a unique challenge: dealing with the coexistence of nascent (unprocessed) and mature (processed) messenger RNA (mRNA). This mix creates hurdles in accurately mapping …
Unraveling RNA sequencing challenges: a new approach to understanding nascent and mature mRNA
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