Spatial total RNA-sequencing shows role of elusive RNA in muscle regeneration
In recent years, scientists who study gene expression in cells have used a method that…
Browsing: Workflows
Unique molecular identifiers and multiplexing amplicons maximize the utility of deep sequencing to critically assess population diversity in RNA viruses
Next generation sequencing (NGS)/deep sequencing has become an important tool in the study of viruses.…
Single-molecule counting method for digital quantification of SARS-CoV-2 RNA
Digital counting individual nucleic acid molecule is of great significance for fundamental biological research and…
FusionSeeker – gene fusion detection and characterization in long-read cancer transcriptome sequencing data
Gene fusions are prevalent in a wide array of cancer types with different frequencies. Long-read…
HT-smFISH – a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging
The ability to visualize RNA in its native subcellular environment by using single-molecule fluorescence in…
Mathematical model could bring us closer to effective stem cell therapies
The model could allow scientists to finally figure out the order of genetic operations inside…
INRI-seq – global cell-free analysis of translation initiation and off-target effects of antisense inhibitors
Ribosomes are the “protein factories” of the cell; this is where translation of the nucleotide…
Light-Seq – turning the spotlight on cells in tissues so RNA can tell their story
Full-transcriptome sequencing of hard-to-access cells in intact tissues facilitates deeper understanding of disease and biology…
Remarkable improvement in detection of readthrough downstream-of-gene transcripts by semi-extractable RNA-sequencing
The mammalian cell nucleus contains dozens of membrane-less nuclear bodies that play significant roles in…
SHERRY2 – a specifically optimized protocol for scRNA-seq without second strand cDNA synthesis
Prevalent single cell transcriptomic profiling (scRNA-seq) mechods are mainly based on synthesis and enrichment of…