Droplet vs picowell – considerations for single-cell transcriptomic profiling of human colon biopsies

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Advances in RNA sequencing have opened new frontiers in understanding how individual cells behave in healthy and diseased tissues. This is especially important for studying the human gastrointestinal (GI) tract, where diseases like colorectal cancer and inflammatory bowel disease often originate in epithelial cells—the cells lining the gut. These cells are critical for maintaining …

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