Bacterial cells, unlike mammalian cells, do not have mRNA polyadenylated tails, which presents a challenge when trying to isolate mRNA for study, especially in single-cell RNA sequencing (scRNA-seq). This issue is made more difficult because of the high abundance of ribosomal RNA (rRNA) that can overwhelm the detection of mRNA. To address this problem, …
RiboD-PETRI – an improved bacterial single-cell RNA-seq reveals biofilm heterogeneity
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